Paracoccidioides Spp.: Virulence Factors and Immune-Evasion Strategies

Table 1

Relative content of the main polysaccharides present in the yeast cell wall of different strains of Paracoccidioides brasiliensis. Strains belonging to at least three different cryptic species were grown at 37°C on RPMI 1640 (Gibco) liquid medium, buffered with 0.165 M morpholinepropanesulfonic acid (MOPS) to pH 7.0 for 4 days.

Morphological phase	Polysaccharide	Polysaccharide content per P. brasiliensis strain (cryptic species)
		Pb73 (PS3)	Pb300 (PS4)	Pb377 (PS4)	Pb444 (PS4)	Pb381 (S1)
M	α-(1,3)-Glucan	1.06 ± 0.5	tr	tr	tr	7.0 ± 0.3
	β-(1,3)-Glucan	31.4 ± 0.4	25.4 ± 0.1	27.7 ± 0.2	20.2 ± 1.3	22.2 ± 1.1
	Chitin	13.2 ± 0.7	17.3 ± 0.4	12.6 ± 0.6	8.6 ± 0.2	13.5 ± 0.5
Y	α-(1,3)-Glucan	22.4 ± 0.9	23.7 ± 0.2	23.8 ± 0.4	24.1 ± 0.8	32.6 ± 1.0
	β-(1,3)-Glucan	10.6 ± 0.6	6.8 ± 0.5	3.9 ± 0.2	8.6 ± 0.4	6.3 ± 0.3
	Chitin	35.1 ± 1.3	31.4 ± 0.6	18.0 ± 0.2	26.6 ± 0.8	23.5 ± 0.8

tr stands for traces.