Innate Immunity of Adipose Tissue in Rodent Models of Local and Systemic Staphylococcus aureus Infection

Figure 3

Expression and regulation of cathelicidin and TLR2 during adipocyte differentiation and upon stimulation with the TLR2 agonistic lipopeptides Pam3Cys and MALP-2. 3T3-L1 fibroblasts were differentiated into mature adipocytes by applying a hormonal differentiation program. Gene expression analysis was performed during adipocyte differentiation at day 0 (fibroblasts), day 3, day 5, day 7, and day 9 (mature adipocytes). = 4–6 wells were analyzed per group. Box plots are shown indicating the median value and upper and lower quartiles, whiskers (1.5-fold of interquartile range). Outliers are marked by circles and asterisks. TLR2, toll-like receptor-2; Pam3Cys, (S)-(2,3-bis(palmitoyloxy)-(2RS)-propyl)-N-palmitoyl-(R)-Cys-(S)-Ser(S)-Lys4-OH-trihydrochloride (TLR1/2-agonist); MALP-2, macrophage-activating lipopeptide-2 (TLR2/6-agonist). (a) Cathelicidin gene expression during 3T3-L1 adipocyte differentiation. Cathelicidin mRNA expression is given as fold-expression in relation to undifferentiated fibroblasts at day 0 of differentiation. (b) TLR2 gene expression during 3T3-L1 adipocyte differentiation. TLR2 mRNA expression is given as fold-expression in relation to undifferentiated fibroblasts at day 0 of differentiation. (c) Cathelicidin gene expression in mature 3T3-L1 adipocytes upon stimulation with the lipopeptides Pam3Cys and MALP-2. Cathelicidin mRNA expression is given as fold-expression in relation to unstimulated control cells. (d) TLR2 gene expression in mature 3T3-L1 adipocytes upon stimulation with the lipopeptides Pam3Cys and MALP-2. TLR2 mRNA expression is given as fold-expression in relation to unstimulated control cells. (e) Cathelicidin gene expression in 3T3-L1 fibroblasts upon stimulation with the lipopeptides Pam3Cys and MALP-2. Cathelicidin mRNA expression is given as fold-expression in relation to unstimulated control cells. (f) TLR2 gene expression in 3T3-L1 fibroblasts upon stimulation with the lipopeptides Pam3Cys and MALP-2. TLR2 mRNA expression is given as fold-expression in relation to unstimulated control cells. “” and “” indicate outliers in the respective data sets displayed as box plots.

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