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Mediators of Inflammation
Volume 2017, Article ID 5380638, 9 pages
https://doi.org/10.1155/2017/5380638
Clinical Study

A Double-Blinded Randomized Study Investigating a Possible Anti-Inflammatory Effect of Saxagliptin versus Placebo as Add-On Therapy in Patients with Both Type 2 Diabetes And Stable Coronary Artery Disease

1Center for Clinical Heart Research, Department of Cardiology, Oslo University Hospital Ullevaal, Oslo, Norway
2Center for Heart Failure Research, Oslo University Hospital Ullevaal, Oslo, Norway
3Faculty of Medicine, University of Oslo, Oslo, Norway
43rd Medical Department for Cardiology, Wilhelminenspital, Vienna, Austria
5The Blood Cell Research Group, Department of Medical Biochemistry, Oslo University Hospital Ullevaal, Oslo, Norway

Correspondence should be addressed to Ida Unhammer Njerve; on.oiu.nisidem@evrejn.u.i

Received 24 November 2016; Revised 31 March 2017; Accepted 6 April 2017; Published 17 May 2017

Academic Editor: Julio Galvez

Copyright © 2017 Ida Unhammer Njerve et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Promising results regarding potential anti-inflammatory and antiatherosclerotic effects of gliptins have been reported. Our aim was to investigate whether saxagliptin treatment modifies expression of inflammatory markers, primarily in peripheral blood mononuclear cells (PBMCs) and in circulating leukocytes in patients with stable coronary artery disease (CAD) and T2DM. Methods. Patients () were randomized to saxagliptin 5 mg daily or placebo for 3 months. Samples were taken at baseline and end of study in fasting state prior to intake of medications. PBMCs were isolated and cryopreserved at −150°C until ex vivo exposed to 1 ng/mL of lipopolysaccharide (LPS) for 4 hours. Gene expression was performed with custom-designed TaqMan® Arrays and relative quantification by real-time PCR (RT-qPCR). Results. HbA1c was reduced in the saxagliptin-treated group compared to that in the change with placebo (). In unstimulated PBMCs and in circulating leukocytes, we observed a significant increase in IL-10 expression in the saxagliptin group (, both), significantly different from that in the placebo ( and , resp.). No between group differences in changes were observed in any of the selected proinflammatory markers. Conclusion. In our small cohort of patients with combined T2DM and CAD, a possible anti-inflammatory effect of saxagliptin, observed in the present study by upregulation of IL-10 in leukocytes, needs to be confirmed in larger studies.