Key Role of STAT4 Deficiency in the Hematopoietic Compartment in Insulin Resistance and Adipose Tissue Inflammation

Figure 4

Glucose tolerance, insulin resistance, and islet insulin secretion. Rag1−/− controls and Rag1−/− mice adoptively transferred with either STAT4−/− CD4+ or CD8+ or with STAT4+/+ CD4+ or CD8+ cells were fed a high fat diet for 15 weeks. Intraperitoneal glucose tolerance test (GTT) (a) and insulin tolerance test (ITT) (b) were performed and area under curve (AUC) was measured for the GTT. Plotted values in the histogram are average ± SEM from –12 mice/group. Statistical analysis was performed using one-way ANOVA. (c) In vitro glucose stimulated insulin secretion was measured in pancreatic islets in mice after 15 weeks on HFD. Islets were cultured overnight and subsequently treated for 1 hr with 3 mM or 18 mM glucose. Insulin was measured by ELISA in the media of the islets stimulated with low or high glucose concentration. Results represent average ± SEM from mice/group; the null hypothesis was rejected for a value < 0.05.

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