Key Role of STAT4 Deficiency in the Hematopoietic Compartment in Insulin Resistance and Adipose Tissue Inflammation

Figure 5

Immune composition and inflammation in epididymal adipose tissue of Rag1−/− mice following adoptive transfer and HFD. (a) Flow cytometry analysis of leukocyte and CD4+ T cells in adipose tissue stromal vascular fraction (SVF) and spleen of Rag1−/− mice following adoptive transfer with STAT4+/+ CD4+ or with STAT4−/− CD4+ cells. The number of cells was counted in the CD45+ gate and the double immunopositive CD3+CD4+ population was measured within the CD45+ gate. (b) Representative FACS plots illustrating the general gating strategy. (c) Same flow cytometry protocol was applied to determine the total number of leukocytes and Cd3+CD8+ positive cells in SVF and spleens of Rag1^−/− mice adoptively transferred with STAT4+/+ CD8+ or with STAT4−/− CD8+ cells. Histograms in (a) and (c) represent mean ± SEM from 5–7 mice/group. (d) Real-time PCR chemokine gene expression in pancreatic islets of STAT4+/+ CD8+ and STAT4−/− CD8+ mice after 15 weeks of HFD (/group). (e) Real-time PCR gene expression of cytokines and chemokines of adipocytes isolated from epididymal adipose tissue of adoptively transferred mice following 15 weeks of HFD ( mice/group); .

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