Aloperine Protects Mice against DSS-Induced Colitis by PP2A-Mediated PI3K/Akt/mTOR Signaling Suppression

Figure 7

PP2A plays a role in aloperine-induced inhibition of T-cell and lymphocyte proliferation and promotion of the expression of Foxp3. (a) Cell viability was assessed in the mixed lymphocyte reaction (MLR) of a mouse by CCK-8 assay. (b, c, and d) Cell viability of Jurkat cells, mouse splenic mononuclear cells, and human splenic mononuclear cells after 24 or 48 h of aloperine and LB-100 treatments. (e) Western blotting analysis of Foxp3 in mouse T-cells following 72 h treatment with aloperine (0.25 mM) and LB-100 (4 μM). (f) Western blotting analysis of p-mTOR, p-Akt, and Foxp3 in mouse Tregs following 24 h treatment with aloperine (0.25 mM) and LB-100 (4 μm). The data are shown as mean ± SEM from three independent experiments. , , and .

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