Different Modulatory Effects of IL-17, IL-22, and IL-23 on Osteoblast Differentiation

Figure 2

Gene expression levels of IL-17, IL-22, and IL-23 receptors in different osteoblast (OB) models. (a–d) The gene expression levels of IL-17, IL-22, and IL-23 receptors in primary osteoblasts, C2C12, MC3T3-E1, and Saos-2 were determined by RT-PCR analysis after the cells were cultured in the absence or presence of BMP-2 (300 ng/ml); GAPDH was used as a gel loading control. (e–h) The gene expression levels of IL-17, IL-22, and IL-23 receptors in primary osteoblasts, C2C12, MC3T3-E1, and Saos-2 were analyzed by quantitative real-time RT-PCR; the copies of the receptor mRNA was standardized with the copies of GAPDH mRNA.

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