Different Modulatory Effects of IL-17, IL-22, and IL-23 on Osteoblast Differentiation

Figure 5

The effects of IL-17 on ALP activity and ALP mass of primary osteoblasts. (a) ALP activity in primary osteoblasts cultured with BMP-2 (300 ng/ml) in the presence of IL-17 (25 ng/ml), IL-22 (25 ng/ml), or IL-23 (25 ng/ml) for 7 days. ^∗∗∗ compared with untreated control; ^# compared with the cells stimulated with BMP-2 alone. (b) Staining for ALP in primary osteoblasts cultured with BMP-2 (300 ng/ml) in the presence of IL-17 (25 ng/ml), IL-22 (25 ng/ml), or IL-23 (25 ng/ml) for 7 days. (c) ALP activity in primary osteoblasts cultured with BMP-2 (300 ng/ml) in the presence of different concentrations of IL-17 (0, 5, 25, and 125 ng/ml) for 7 days. ^∗∗ compared with untreated control; ^# compared with the cells stimulated with BMP-2 alone. (d) Staining for ALP in primary osteoblasts cultured with BMP-2 (300 ng/ml) in the presence of different concentrations of IL-17 (0, 5, 25, and 125 ng/ml) for 7 days.

(a)

(b)

(c)

(d)