The Antimicrobial Peptide Human Beta-Defensin-3 Is Induced by Platelet-Released Growth Factors in Primary Keratinocytes

Figure 3

The epidermal growth factor receptor (EGFR) partially mediated the hBD-3 gene induction in keratinocytes stimulated with PRGF. To analyze the influence of the EGFR on the PRGF-mediated hBD-3 induction, human primary keratinocytes were treated with PRGF (1 : 10 diluted in cell culture medium) in the presence or absence of the EGFR-blocking antibody cetuximab. HBD-3 gene expression was analyzed by real-time PCR (, ns = not significant, Student’s t-test).