The Antimicrobial Peptide Human Beta-Defensin-3 Is Induced by Platelet-Released Growth Factors in Primary Keratinocytes

Figure 4

IL-6 signaling seems to play only a marginal role for the observed PRGF-induced hBD-3 expression. We stimulated primary human keratinocytes with PRGF (1 : 10 diluted in cell culture medium) and used the IL-6 receptor-blocking antibody tocilizumab (50 μg/ml) to analyze the influence of IL-6 signaling on PRGF-mediated hBD-3 gene induction. HBD-3 gene expression was analyzed by real-time PCR (, ns = not significant, Student’s t-test).