MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression

Figure 1

The severe asthma mice model was established. (a) Pulmonary resistance in the saline, conventional, and severe groups. When Mch was inhaled, the RL of the conventional and severe groups showed a rise, and the RL values of the severe group were higher than those of the conventional group. (b) The total, NEU, and EOS cells of BALF from the three groups. The total and NEU cells of BALF from the severe group were higher than those from the conventional group, but EOS cells showed an opposite result. (c) Lung tissues were stained with H&E, immunohistochemistry (neutrophil-specific antibody (anti-Gr1), eosinophil antibody (anti-ECP)) of the three groups. Histological analyses of lungs from the severe group exhibited markedly enhanced peribronchial inflammation with infiltrated neutrophils, but eosinophils were not obviously different compared with the conventional group. (d) Western blot analysis detected NEU and EOS protein expression in the three groups. The NEU protein expression of the lungs from the severe group were higher than those from the conventional group, whereas EOS protein expression values were not obviously different between them. as compared with the control group. ^# as compared with the conventional group.

(a)

(b)

(c)

(d)