MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression

Figure 4

IL-17 expression and Th17 cell differentiation under MBD2 gene silencing or overexpression. (a) Under MBD2 gene silencing (M(−)), IL-17 and MBD2 protein expression was significantly lower than that of the empty transfection group (M(0)) by Western blot analyses; with MBD2 gene overexpression (M(+)), IL-17 and MBD2 protein expression was markedly increased than that of M(0). (b) Under MBD2 gene silencing (M(−)), Th17 cell differentiation was significantly lower than that of M(0) according to flow cytometry analyses; with MBD2 gene overexpression (M(+)), Th17 cell differentiation was markedly increased compared to that of M(0).

(a)

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