Intranasal Delivery of miR-146a Mimics Delayed Seizure Onset in the Lithium-Pilocarpine Mouse Model

Figure 1

(a) Dynamic changes in relative miR-146a levels in the hippocampus after intranasal delivery of miR-146a mimics. Based on real-time qPCR, the relative miR-146a levels in the hippocampus were gradually upregulated at 15 min and 30 min after intranasal delivery of miR-146a mimics compared with the vehicle control sacrificed at 0 min and then began to decrease at 1 h compared with the 3.69-fold peak at 30 min. . (b) Effectiveness of the lithium-pilocarpine protocol. While seizures began within 1 h after the administration of pilocarpine based on the Racine scale, tonic-clonic discharges were synchronously recorded in all three mice examined using the EEG/EMG acquisition system. (c) Graphic procedure of drug delivery and time of sacrifice in the subsequent experiments.

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