Metformin Suppressed CXCL8 Expression and Cell Migration in HEK293/TLR4 Cell Line

Figure 3

Metformin inhibited LPS-induced NF-κB translocation. HEK294/TLR4 cells were treated with or without metformin for 2 h, followed by 15 min induction with 1 μg/ml LPS. Untreated cells were used as a control. After fixation and permealization steps, cells were stained with antibody against subunit p65 NF-κB and counterstained with DAPI. (a, b, c) Cells without treatment; (d, e, f) cells treated with 1 μg/ml LPS; (g, h, i) cells treated with 10 mM metformin and 1 μg/ml LPS. (a, d, g) anti-p65 antibody; (b, e, h) DAPI counterstaining; (c, f, g) merged image p65/DAPI.