Growth Arrest-Specific 6 Enhances the Suppressive Function of CD4⁺CD25⁺ Regulatory T Cells Mainly through Axl Receptor

Figure 1

TAM receptors were expressed in mouse CD4⁺CD25⁺Tregs. (a) Protein expression from CD4⁺CD25⁺Tregs, cardiac tissue, and kidney tissue was analyzed by Western blot using specific anti-Axl (2 μg/ml) and anti-Mertk (2 μg/ml) antibodies. (b) Axl and Mertk mRNA expressions in CD4⁺CD25⁺Tregs and marcrophages were determined by semiquantitative RT-PCR. Expression of β-actin was shown as an internal control. (c) CD4⁺CD25⁺Tregs were incubated with anti-Axl (10 μg/ml) and anti-Mertk (10 μg/ml) monoclonal Ab, respectively, followed by FITC-labeled goat anti-rat IgG as the secondary Ab (1 : 30). The cells were analyzed by flow cytometry.

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