Growth Arrest-Specific 6 Enhances the Suppressive Function of CD4⁺CD25⁺ Regulatory T Cells Mainly through Axl Receptor

Figure 6

Gas6 modulates the Foxp3 and CTLA4 expression mainly through Axl receptor. (a–d) CD4⁺CD25⁺Tregs were treated with anti-Axl or anti-Mertk Abs or PBS in the presence of 100 ng/ml rmGas6. After 24 h of incubation, the expression of CTLA-4 and Foxp3 was determined by flow cytometry (/group). compared with the value for the Gas6 group, and compared with the value for rmGas6+anti-Mertk group. (e–h) The expression of CTLA-4 and Foxp3 in Tregs with or without Axl knockout was determined by flow cytometry. compared with the value for the Gas6 group.

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