Morita-Baylis-Hillman Adducts Display Anti-Inflammatory Effects by Modulating Inflammatory Mediator Expression in RAW264.7 Cells

Figure 4

Effect of A2CN, A3CN, and A4CN on ROS production by RAW264.7 macrophage cells stimulated with LPS (1 μg/mL) for 22 h of incubation. CTR means cells without LPS and MBHA compound incubation. LPS, cells incubated with LPS (1 μg/mL). Cells were labeled with H2-DCFH-DA, and ROS production was quantified by flow cytometry in FL-1 channel. Results represented mean ± SEM of three experiments in triplicate. Data were analyzed by ANOVA, followed Newman-Keuls post hoc test. ^###, compared to CTR group. , , and , compared to LPS + MBHA compound groups.