Associations of Functional MicroRNA Binding Site Polymorphisms in IL23/Th17 Inflammatory Pathway Genes with Gastric Cancer Risk

Figure 5

The relative luciferase activity in six groups. . NC: empty vector; IL17A-WT: IL17A-UTR-WT vector; IL17A-WT + MI-NC: IL17A-UTR-WT vector transfected with negative control mimic; IL17A-WT + MI: IL17A-UTR-WT vector transfected with miR-10a-3p mimic; IL17A-WT + IN-NC: IL17A-UTR-WT vector transfected with nonspecific inhibitor; IL17A-WT + IN: IL17A-UTR-WT vector transfected with miR-10a-3p inhibitor. The relative luciferase was assessed by luciferase report gene assay. Data was evaluated statistically using -test and represent the mean ± standard deviation from the experiments in triplicate.