Anti-Inflammatory Activity of Crude Venom Isolated from Parasitoid Wasp, Bracon hebetor Say

Figure 1

B. hebetor venom suppressed LPS-mediated NO release without any cytotoxicity. RAW 264.7 cells were seeded in 96-well plates for 18 hr and then preincubated with BHV (0.1 μg/mL–0.4 μg/mL) for 30 min and then stimulated with LPS (0.1 μg/mL) for 18 hr. The cell supernatant was transferred to a 96-well plate and reacted with equal amounts of Griess reagent, and then NO production was measured at 540 nm (a). Effects of BHV on cell viability were measured by MTT assay and absorbance was measured at 560 nm (b). NO production was also observed in mice chronically treated with LPS (c). For survival rate, mice were given LPS injection with a lethal dose of 30 μg/kg and monitored for 96 hours (d). Values in bar graph are mean ± SEM of at least 3 independent experiments. compared to LPS only.

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