Anti-Inflammatory Activity of Crude Venom Isolated from Parasitoid Wasp, Bracon hebetor Say

Figure 2

Inhibitory effects of B. hebetor venom on the expression of iNOS and COX-2. For mRNA and protein expressions, RAW 264.7 cells were seeded in 6-well plates and treated with indicated concentrations of BHV (0.1 μg/mL–0.4 μg/mL) for 30 min and then stimulated with LPS (0.1 μg/mL) for 18 hr. Total RNA from cell, lung, and testis tissues and total protein was extracted using their respective kit protocols. PCR product was analyzed on agarose gels with GAPDH as the internal control. Protein quantitation was done by Pro-Measure (iNtRON biotechnology, Daejeon, Korea), and then proteins were run on SDS-PAGE and analyzed by ECL chemiluminisence with β-actin as internal control. PCR and protein expression in RAW 264.7 cells (a-b). PCR and protein expression in lungs and testis tissue (c-d). Western blot and PCR images are representative of 3 independent experiments. Bar values of , , and were considered statistically significant. BL = basal lung; BT = basal testis; LL = LPS lung; LT = LPS testis; LV = lung venom; TV = testis venom.

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