Anti-Inflammatory Activity of Crude Venom Isolated from Parasitoid Wasp, Bracon hebetor Say

Figure 5

NF-κB and MAPK pathways are followed by B. hebetor venom. RAW 264.7 cells were pretreated with BHV at the indicated concentrations (0.1 μg/mL–0.4 μg/mL) and then stimulated with or without LPS (0.1 μg/mL) for 18 hr. Nuclear and cytosolic proteins from cells and tissues were extracted using NE-PER extraction kit. Proteins were run on SDS-PAGE and treated with primary antibodies overnight followed by incubation with secondary antibodies for 2 hr. Expression of proteins in cells (a). Expression of proteins in lung and testis tissue (b). MAPK pathway proteins expression (c). The membranes were analyzed by ECL chemiluminescence system. Data are mean ± SD (); and compared with LPS only. β-actin was taken as internal control.

(a)

(b)

(c)