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Mediators of Inflammation
Volume 2017, Article ID 7043429, 12 pages
Research Article

A Reduction in Selenoprotein S Amplifies the Inflammatory Profile of Fast-Twitch Skeletal Muscle in the mdx Dystrophic Mouse

1Institute for Physical Activity and Nutrition Research (IPAN), School of Exercise and Nutrition Sciences, Deakin University, Geelong, VIC, Australia
2Molecular Medical Research SRC, School of Medicine, Deakin University, Geelong, VIC, Australia
3Department of Medicine, The University of Melbourne, Melbourne, VIC, Australia

Correspondence should be addressed to Craig Robert Wright; ua.ude.nikaed@thgirw.giarc

Received 29 November 2016; Revised 23 February 2017; Accepted 7 March 2017; Published 16 May 2017

Academic Editor: Mirella Giovarelli

Copyright © 2017 Craig Robert Wright et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

TABLE 1: Mouse Primers Used for qPCR. Cluster of differentiation 68 (CD68), cluster of differentiation 163 (CD163), EGF-like module-containing mucin-like hormone receptor-like 1 (F4/80), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), glucose-regulated protein 78 (Grp78), interleukin 1β (IL-1Β), inducible nitric oxide (iNOS), monocyte chemoattractant protein 1 (MCP-1), myeloperoxidase (MPO), transforming growth factor Β1 (TGF-Β1),tumour necrosis factor a (TNFa). SUPPLEMENTARY FIGURE 1: Ex vivo strength and fatigue analysis of the soleus. (a) Force Frequency curve of the soleus muscle in response to ex vivo stimulation, and (b) specific force production of the soleus during a 4 minute submaximal fatiguing stimulation, and force recovery at 2, 5 and 10 minutes (n⁼11). SUPPLEMENTARY FIGURE 2: Inflammatory gene profile of the soleus. (a) Monocyte chemoattractant protein 1 (MCP-1), (b) EGF-like module-containing mucin-like hormone receptor-like 1 (F4/80), (c) transforming growth factor β1 (TGF-Β1), (d) interleukin 6 (IL-6), (e) interleukin 1β (IL-1Β) and (f) tumour necrosis factor a (TNFa) gene expression in the soleus muscle at 12 weeks of age. Data are represented as fold change ± SEM, and are normalised to GAPDH. No differences in GAPDH expression were present between groups (n⁼11).

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