Research Article

Osteopontin Augments M2 Microglia Response and Separates M1- and M2-Polarized Microglial Activation in Permanent Focal Cerebral Ischemia

Table 1

Details of primary antibodies.

AntibodyAntigeneDilution/fixationIdentified cell typeReference, company

Anti-NeuNNeuronal nuclear antigen (DNA-binding)1 : 2000 4% PFA,
(2 h)
Surviving neuronsAnti-NeuN monoclonal antibody, mouse, catalog number MAB377, Merck Millipore
Anti-Iba1Ionized calcium binding adaptor molecule 11 : 1000, 4% PFA,
(2 h)
Activated microglia/macrophagesAnti-iba1 polyclonal antibody, rabbit, catalog number 019-19741, Wako Chemicals
Anti-iNOSInducible NO-synthetase1 : 100, 4% PFA,
(15 min)
M1-polarized microgliaAnti-iNOS polyclonal antibody, rabbit, catalog number ab15323, abcam
Anti-Arg1Arginase 11 : 1000 4% PFA,
(15 min)
M2-polarized microgliaAnti-liver-arginase polyclonal antibody, goat, catalog number ab60176, abcam
Anti-CD206Mannose receptor1 : 1500, 4% PFA,
(30 min)
M2-polarized microgliaAnti-CD206 polyclonal antibody, goat, catalog number AF2535, R&D Systems
Anti-Ym1Chitinase-3-like-31 : 100, 50% acetone
(15 min, 4°C) 0.3% TX
M2-polarized microgliaAnti-Ym1 polyclonal, rabbit, catalog number 01404, Stemcell Technologies
Anti-vWFvon Willebrand factor1 : 1500, 4% PFA,
(15 min)
Endothelial cellsAnti-vWF polyclonal antibody, rabbit, catalog number ab6994, abcam
Anti-NG2Chondroitin sulfate proteoglycan1 : 4000, 100% acetone
(10 min, −20°C)
PolydendrocytesAnti-NG2 polyclonal antibody, rabbit, catalog number AB5320, Millipore
Anti-GFAPGlial fibrillary acidic protein1 : 2000, 4% PFA
(overnight, 4°C)
AstrocytesAnti-GFAP monoclonal antibody, mouse, catalog number MAB360, Millipore