Figure 7: Compared to WT animals, LTβR−/− animals show delayed increase of TNFα in the serum in the acute phase of infection with T. gondii. 50 μL of murine serum was collected from uninfected and infected WT and LTβR−/− animals, TNFα levels were determined by ELISA (a), and nitric oxide levels were determined by colorimetric detection of nitrite after conversion of nitrate to nitrite (b). ( in all cases except d 0 (both genotypes) and d 14 (LTβR−/−), where only 2 animals were analyzed). , .