HaCaT Cells as a Reliable In Vitro Differentiation Model to Dissect the Inflammatory/Repair Response of Human Keratinocytes

Figure 5

In vitro release of cytokines, chemokines, and growth factors from primary adult human keratinocytes stimulated by TNFα. The amount of chemokines (a), of cytokines, and of growth factors (b) was measured by Multiplex system technology in the supernatants of primary adult human keratinocytes (pKC) plated at the same density (8–10 × 10⁴ cells/cm²) and grown in low Ca²⁺-containing medium (white bars). The treatment with 10 ng/ml TNFα was carried out for 48 hours in a serum-free medium when cells were 60–70% confluent (black bars). The data are expressed as pg/10⁶ cells.

(a)

(b)