TSG-6 Downregulates IFN-Alpha and TNF-Alpha Expression by Suppressing IRF7 Phosphorylation in Human Plasmacytoid Dendritic Cells

Figure 2

TSG-6 reduced the transcription of interferon regulatory factor 7 and its associated genes in GEN2.2. TSG-6 reduced the transcription of interferon regulatory factor 7 and its associated genes in GEN2.2. Real-time PCR analysis of IRF7, IFN-α2, TNF-α, and IL-1β in GEN2.2 cells treated with TSG-6 (1000 ng/mL) overnight, followed by stimulation with CpG-A at 2 μM (a) or R837 at 10 ng/mL (b) for 3 hours. Graphs show data of more than 3 experiments performed in duplicate. Error bars indicate SEM. One-Way ANOVA, , .

(a)

(b)