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Figure 5: TSG-6 increased CD44 expression. Real-time PCR (a and b) and flow-cytometric analysis (c and d) of GEN2.2 cells treated with TSG-6 (1000 ng/mL), followed by stimulation with CpG-A (2 μM) or R837 (10 ng/mL) for 3 hours. Representative FACS plots showing CD44+ pDCs percentages per independent experiment (). Graphs showing data of at least 3 experiments performed in duplicate. Error bars indicate SEM. One-Way ANOVA, , .