TSG-6 Downregulates IFN-Alpha and TNF-Alpha Expression by Suppressing IRF7 Phosphorylation in Human Plasmacytoid Dendritic Cells

Figure 7

TSG-6 exerted the same suppressive effect on pDCs from healthy donors stimulated with CpG-A or R837. PBMC from 14 healthy blood donors were isolated and treated with TSG-6 (1000 ng/mL) overnight, followed by CpG-A (2 μM) (a) or R837 (10 ng/mL) (b) for 5 hours. The cells were stained with the cell surface markers as indicated, fixed, and permeabilized, followed by staining with APC-conjugated anti-IFN-α and FITC-conjugated anti-TNF-α antibodies. PBMCs were gated for pDCs as defined in the gating strategy (Supplementary data S2). MFI, mean fluorescence intensity. Error bars indicate SEM. Wilcoxon matched-pairs signed rank test was used to determine statistical significance. , .

(a)

(b)