Illicium verum Extract and Trans-Anethole Attenuate Ovalbumin-Induced Airway Inflammation via Enhancement of Foxp3+ Regulatory T Cells and Inhibition of Th2 Cytokines in Mice
Table 1
Quantification of immune cell subtypes in lungs and BALF by FACS analysis.
Cell phenotypes in lungs and BALF
Normal Balb/c mice
OVA-induced asthma mice (total absolute number)
Control
CsA (10 mg/kg)
IVE (200 mg/kg)
AET (20 mg/kg)
AET (2 mg/kg)
Lung
CD4+/CD3+ (1 × 106 cells)
2.6 ± 0.77
9.6 ± 0.18
3.6 ± 0.44
3.2 ± 0.32
4.9 ± 0.29
8.3 ± 0.23
B220+/CD23+ (1 × 106 cells)
0.5 ± 0.09
7.1 ± 0.77
1.2 ± 0.18
1.4 ± 0.02
2.6 ± 0.23
4.8 ± 0.34
CD8+/CD3+ (1 × 106 cells)
1.7 ± 0.43
4.1 ± 1.14
1.9 ± 0.09
1.7 ± 0.19
2.3 ± 0.04
3.5 ± 0.21
CD69+/CD3+ (1 × 105 cells)
0.7 ± 0.06
7.3 ± 0.23
2.7 ± 0.20
2.7 ± 0.15
4.0 ± 0.52
4.7 ± 0.29
CD11b+/Gr-1+ (1 × 106 cells)
0.6 ± 0.00
7.2 ± 0.55
2.3 ± 0.30
2.7 ± 0.18
3.9 ± 0.14
4.5 ± 0.74
CCR3+/CD3− (1 × 105 cells)
0.7 ± 0.02
13.5 ± 1.05
3.3 ± 0.46
3.9 ± 0.05
6.0 ± 0.01
8.1 ± 2.72
BALF
CD4+/CD3+ (1 × 106 cells)
0.5 ± 0.29
30.5 ± 5.20
6.4 ± 1.93
6.6 ± 1.86
7.8 ± 0.03
30.6 ± 3.81
CD11b+/Gr-1+ (1 × 106 cells)
0.4 ± 0.29
15.1 ± 4.03
3.4 ± 0.48
4.6 ± 0.66
3.8 ± 1.03
8.2 ± 2.53
CCR3+/CD3− (1 × 105 cells)
0.2 ± 0.09
13.5 ± 1.42
2.0 ± 0.41
4.5 ± 1.28
3.8 ± 0.39
9.9 ± 0.13
Results are expressed as mean ± SEM ( mice per group). , , and indicate statistically significant differences between the asthma control group and drug-treated group.
