Osteopontin Impacts West Nile virus Pathogenesis and Resistance by Regulating Inflammasome Components and Cell Death in the Central Nervous System at Early Time Points

Figure 2

OPN levels correlate with WNV-infected cells in WT brains. Viral peptide WNV-E and OPN were detected in the brain of WT-infected animals 3 and 5 days following infection. (a) Detection of OPN transcripts in the brain of WT (open circles) and OPN KO (closed circles) animals at baseline, 3 and 5 dpi. Immunohistochemistry staining of WT animal brain frontal cortex for detection of WNV-E envelope (b) at 3 dpi and (c) at 5 dpi and of OPN protein (d) at 3 dpi and (e) at 5 dpi. Results represent the mean ± SEM of 6 animals per group. , two-way ANOVA followed by Bonferroni’s test.