Anti-Inflammatory and Neuroprotective Role of Natural Product Securinine in Activated Glial Cells: Implications for Parkinson’s Disease

Figure 4

Securinine attenuates LPS-induced activation of NF-κB in BV2 microglia. (a) BV2 microglial cells were treated with securinine for 1 h followed by stimulation with 500 ng/mL LPS. After 30 min of LPS stimulation, isolated nuclear lysates were subjected to Western blotting to assess translocation of phosphor-65 protein to the nucleus. PARP was used as a nuclear loading control. (b) BV2 cells were transiently transfected with NF-κB-Luc plasmid for 24 h and then treated with 500 ng/mL LPS for 4 h ± securinine. Whole cell lysates were assayed via the luciferase activity (mean ± SE, ); versus control. versus LPS. versus LPS.

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