Anti-Inflammatory and Neuroprotective Role of Natural Product Securinine in Activated Glial Cells: Implications for Parkinson’s Disease

Figure 6

Securinine pretreatment reduces IFN-γ-induced NO production, iNOS mRNA expression, and phosphorylation of STAT1α in BV2 microglia. BV2 microglial cells were pretreated with securinine for 1 h and then challenged with IFN-γ (50 U/mL) for 24 h (a), 6 h (b), or 30 min (c). The expression levels of nitric oxide, iNOS mRNA, and tyrosine phosphorylation of STAT1α were determined by Griess Reagent assay (a), real-time PCR analysis (b), or Western blotting (c), respectively. The data are expressed as mean ± SD, . significantly different from control samples. , significantly different from the LPS-treated group.

(a)

(b)

(c) ⁡