Anti-Inflammatory and Neuroprotective Role of Natural Product Securinine in Activated Glial Cells: Implications for Parkinson’s Disease

Figure 7

Securinine protects primary mesencephalic dopaminergic neurons through inhibition of BV2 cells activation. BV2 cells were stimulated with LPS (500 ng/mL) ± securinine (10 μM) for 24 h. CM from control (BV2-CM), securinine-treated (Sec-CM), LPS-treated (LPS-CM), and LPS/securinine-treated (5 and 10 μM; LPS + Sec 5 μM-CM, LPS + Sec 10 μM-CM) BV2 cells was added to primary mesencephalic cultures. After 72 h, cultures were immunostained for TH⁺ neurons (a) and the number of TH⁺ neurons was counted (b). Data are expressed as mean ± SD, ; , compared with control-CM group, , compared with LPS-CM group.

(a)

(b)