Anti-Inflammatory and Neuroprotective Role of Natural Product Securinine in Activated Glial Cells: Implications for Parkinson’s Disease

Figure 8

Securinine pretreatment inhibits LPS-induced NO production in RAW 264.7 macrophage cells and in primary astrocyte cultures. RAW 264.7 macrophage cells or primary astrocyte cultures (10 × 10⁴ cells/well in 48-well plates) were incubated with 500 ng/mL of LPS or LPS/IFN-γ (50 unit/mL) in the presence or absence of securinine (10 μM) for 24 h. The amount of nitrite secreted into the supernatant was measured using the Griess Reagent assay ((a) RAW 264.7 cells, versus control; (b and c) primary astrocyte cultures). The data were expressed as the mean ± SD () and are representative of three independent experiments. and , significantly different from cells treated with LPS only.

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