NLRP3 Deficiency Attenuates Renal Fibrosis and Ameliorates Mitochondrial Dysfunction in a Mouse Unilateral Ureteral Obstruction Model of Chronic Kidney Disease

Figure 7

NLRP3 deletion reverses UUO-induced mitochondrial dysfunction. Representative electron microscopy photomicrographs of ultrastructural morphology of mitochondria (a), (magnification ×12,000). Arrow indicates swollen mitochondria. Expression of PGC-1a in kidney sample was detected, and semiquantitative analysis of PGC-1a normalized against β-actin (b). Semiquantitative analysis of mtDNA expression normalized against 18S performed by real-time PCR (c). Data represent the mean ± SEM (). , compared with WT/Sham group, , NLRP3^−/−/U14 group versus WT/U14 group. U1, U3, U7, and U14 indicate that mice were sacrificed on days 1, 3, 7, and 14 after UUO.

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