Syndecan-1 Acts as an Important Regulator of CXCL1 Expression and Cellular Interaction of Human Endometrial Stromal and Trophoblast Cells

Figure 1

Evaluation of the Sdc-1 kd in the human EnS cell lines. (a) Sdc-1 mRNA levels determined by QGP assay [MFI = mean fluorescence intensity], the qualitative Sdc-1 protein expression was determined by dot blot analysis depicted in (b) and the corresponding pixel density evaluation is shown in (c) with and without decidualization (+/−d) and with and without inducing the Sdc-1 kd (+/−Tet) in KdS1 cells with 1 μg/mL Tet. The dot blot analysis was performed with 30 μg protein per dot, and samples were applied in the same order as in (c). Data represent means ± SD with for Tet treated KdS1 cells versus untreated KdS1 (+/−d) cells.

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