A Proinflammatory Effect of the β-Glucan from Pleurotus cornucopiae Mushroom on Macrophage Action

Figure 1

P. citrinopileatus polysaccharide PCPS induces secretion of proinflammatory cytokine, TNF, in THP-1 macrophages. Human monocyte cell line THP-1 was differentiated into macrophages by PMA (160 nM) for 48 hrs., and then they were stimulated with the different concentrations of the 450 kD polysaccharide (PCPS) purified from a hot water extract of P. citrinopileatus fruiting bodies, or LPS (10 ng/ml, as a positive control). After 24 hours, the secretion of TNF in the cell supernatant was determined by ELISA. The data shown are the average of 3 independent experiments. The depicted error bars represent the SEM of these 3 experiments. For statistical analysis, a one-way ANOVA followed by the Dunnett’s t-test was used; and when compared to the RPMI medium control.