A Proinflammatory Effect of the β-Glucan from Pleurotus cornucopiae Mushroom on Macrophage Action

Figure 5

Influences of the P. citrinopileatus extract on cytokine expression in the mice spleen cells. After acclimation for 7 days, the animals were randomly divided into 2 groups ( for each group): normal control (C), P. citrinopileatus extract group (PCPS). The experimental extracts dissolved in distilled water were orally administered every day for two weeks. Normal control mice were administered an equal volume of distilled water. The mRNA expression levels of IFNγ (a) and IL-4 (b) in the mice spleen cells were determined by using quantitative real-time PCR. Expression was determined relative to glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and then each expression level was compared to the level of the normal control group. The depicted error bars represent the SEM, and Student’s t-test was used for the statistical analysis; when compared to the normal control.

(a)

(b)