IL-10-Producing B Cells Suppress Effector T Cells Activation and Promote Regulatory T Cells in Crystalline Silica-Induced Inflammatory Response In Vitro

Figure 1

Insufficient B10 promoted the proinflammatory cytokines in the Teff and B cell coculture system in vitro. (a1) CD19⁺ B cells were purified and checked by flow cytometry. (a2, a3) The percentages of B10 in different groups were tested by flow cytometry. (b1, b2) The CD4⁺CD25⁻ Teff and CD4⁺CD25⁺ Treg were purified and checked by flow cytometry. (c) A schematic of B cell and Teff coculture system with insert plates in vitro. (d, e) The expressions of TNF-α and IL-6 in different groups of the B cell and Teff coculture system with or without insert plates were assayed by real-time PCR. (f, h) The secretions of TNF-α and IL-6 in supernatant of the B cell and Teff coculture system were assayed by CBA. (g, i) The concentrations of TNF-α and IL-6 in supernatant of the B cell and Teff coculture system with insert plates were assayed by CBA. (Data was presented as mean ± SEM (). , significantly different compared with the control B + Teff group. ^#, significantly different compared with the silica B + Teff group.)

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