IL-10-Producing B Cells Suppress Effector T Cells Activation and Promote Regulatory T Cells in Crystalline Silica-Induced Inflammatory Response In Vitro

Figure 3

Insufficient B10 promoted the Th2/Th17 responses in the Teff and B coculture system in vitro. (a, b, c) The expressions of IL-4, IL-13, and GATA3 in different groups of the B cell and Teff coculture system with or without insert plates were assayed by real-time PCR. (d1, d2) The percentages of CD4⁺ IL-17A⁺ Th17 cells in different groups of the B cell and Teff coculture system were studied by flow cytometry. (e, f, g) The expressions of IL-17A, IL-23, and ROR-γt in different groups of the B cell and Teff coculture system with or without insert plates were assayed by real-time PCR. (h, i) The secretions of IL-17A in supernatant of the B cell and Teff coculture system with or without insert plates were assayed by CBA. (Data was presented as mean ± SEM (). , significantly different compared with the control B + Teff group. ^#, significantly different compared with the silica B + Teff group.)

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