Figure 4: Effects of iron on iNOS expression in M1 macrophage. RAW264.7 macrophage cells were incubated with the stimulus, either FAC, IFN-γ, or no stimulus for 24 h. Cells were harvested and analyzed for iNOS (a) by western blot or fixed and stained with antibodies for iNOS (b). Protein expression is represented as fold-change compared to unstimulated macrophages. In each sample group, cell was stained with the red fluorescent-labeled antibody for the targeted protein and the blue fluorescent-labeled DAPI for nucleus. Data are representative images of three (a) or two (b) independent experiments. (uns: unstimulated macrophages; ).