Iron Reduces M1 Macrophage Polarization in RAW264.7 Macrophages Associated with Inhibition of STAT1
Effect of iron on STAT1 activation in M1 macrophages. RAW264.7 macrophage cells were incubated with the stimulus, either FAC, IFN-γ, or no stimulus for 24 h. Cell lysates were prepared, and phosphorylation of STAT1 (p-STAT1) was analyzed by western blot. One representative blot and the densitometric quantification are shown. Data are mean ± SD for three independent experiments. (uns: unstimulated macrophages; ).