HIF1α-Induced Glycolysis Metabolism Is Essential to the Activation of Inflammatory Macrophages

Figure 2

HIF1α decreased mitochondrial oxidation and promotes glycolysis metabolism in macrophages. (a and b) Metabolic respiratory parameters of peritoneal macrophages isolated from the WT mice and Lysm HIF1α lsl mice were measured with the treatment of extracellular flux analyzer: A, oligomycin; B, carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP); C, antimycin A and rotenone. (a) The oxygen consumption rate (OCR) value was measured at the basal level and after the treatment of A, B, and C quantitated on the right panel. Basal OCR was measured before the injection of a, and maximal OCR was calculated by subtracting the nonmitochondrial OCR from the peak OCR after the treatment of B. (b) The extracellular acidification rate (ECAR) value was calculated by the software. (c) The OCR/ECAR ratio was calculated at basal level quantitated on the right panel. Statistical comparisons were made using two-tailed Student’s t-test (a and c). compared with WT mice. All values were presented as mean ± SEM for independent experiments in each group.

(a)

(b)

(c)