HIF1α-Induced Glycolysis Metabolism Is Essential to the Activation of Inflammatory Macrophages

Figure 3

Metabolomics analysis of HIF1α-modified macrophage glycolysis metabolism. (a, b, c, d, e, and f) Peritoneal macrophages were isolated from the WT and Lysm HIF1α lsl mice. An LC-MS/MS system was used to measure the abundance of cellular metabolites. Metabolomics data were analyzed using the MetaboAnalyst website. (a) Heatmap of the intracellular metabolites generated from hierarchical clustering. Red series denoted relative high concentrations and blue series denoted relative low concentrations. (b) 2D PLS-DA score plot. (c) 3D PLS-DA score plot. (d) VIP scores. (e) Overview of metabolite enrichment in HIF1α overexpressed macrophages. (f) Metabolic pathway analysis of HIF1α overexpressed macrophages. (g, h, and i) Relative levels of metabolites in the glycolysis metabolism (g), TCA cycle (h), and pentose phosphate pathways (i). Statistical comparisons were made using two-tailed Student’s t-test (g, h, and i). and , compared with WT mice. All values were presented as mean ± SEM for independent experiments in each group. FBP: fructose 1,6-bisphosphate; F-6-P: fructose-6-phosphate; GADP: glyceraldehyde-3-phosphate; G-6-P: glucose-6-phosphate; PEP: phosphoenolpyruvate; 3-PG: 3-phosphoglycerate.

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