Acinetobacter baumannii Lipopolysaccharide Influences Adipokine Expression in 3T3-L1 Adipocytes
Effect of A. baumannii-LPS on adipokine expression including inflammatory cytokines and chemoattractants in 3T3-L1 adipocytes. Differentiated adipocytes were incubated for 1 h at 37°C with 100 ng/mL A. baumannii-LPS (ATCC 19606), 100 ng/mL E. coli-LPS (O111:B4), or saline and subjected to real-time PCR analyses to determine the mRNA expression levels of several adipokines, including MIP-2, TNF-α, IL-6, MCP-1, TLR4, and eNOS. Adipokine expression is presented as the ratio of viable cytokine expression in adipocytes treated with the different bacterial LPS versus the adipocytes treated with saline. All experiments were performed in triplicates, and the results are presented as the mean ± SD. ; ; (compared to adipocytes without LPS treatment).