Acinetobacter baumannii Lipopolysaccharide Influences Adipokine Expression in 3T3-L1 Adipocytes
Induction of MIP-2 by A. baumannii-LPS in 3T3-L1 adipocytes. The dose-dependent effect of A. baumannii-LPS was examined via the MIP-2 expression in adipocytes. The adipocytes were incubated for 1 h in fresh medium containing 10, 100, or 1000 ng/mL of A. baumannii-LPS (ATCC 19606), (a) E. coli-LPS (O111:B4), or saline and were subjected to real-time PCR analyses for the detection of MIP-2 mRNA. (b) MIP-2 expression in adipocytes was measured at the indicated time points following treatment with 100 ng/mL A. baumannii-LPS (ATCC 19606) or 100 ng/mL E. coli-LPS (O111:B4). The expression of MIP-2 is presented as the ratio of viable MIP-2 expression in adipocytes treated with LPS versus those treated with saline at the indicated time points. All experiments were performed in triplicates, and the results are presented as the mean ± SD. (c) The microscopic images (×280 magnification) of 3T3-L1 adipocytes treated with LPS on day 8 via Oil-Red-O staining. 3T3-L1 adipocytes were differentiated in 35 mm dishes and subsequently stimulated with the LPS derived from several bacterial strains (1000 ng/mL) for 24 h. The morphological changes in adipocytes caused by LPS (1000 ng/mL) were assessed by optical microscopy. ; ; (compared to adipocytes without LPS treatment).