Mediators of Inflammation / 2017 / Article / Fig 4

Research Article

Acinetobacter baumannii Lipopolysaccharide Influences Adipokine Expression in 3T3-L1 Adipocytes

Figure 4

Effect of A. baumannii-LPS on MIP-2 secretion from 3T3-L1 adipocytes. (a) The dose-dependent effect of A. baumannii-LPS on MIP-2 secretion from adipocytes. The 3T3-L1 cells were seeded at a density of 4 × 105 cells/well in 35 mm dishes, and the cells were differentiated into adipocytes in MIX-Diff medium. The adipocytes were then incubated for 72 h in fresh medium containing 300, 1000, and 3000 ng/mL of A. baumannii-LPS (ATCC 19606) or E. coli-LPS (O111:B4) and measured for the level of MIP-2 protein in the culture medium by ELISA. (b) MIP-2 secretion from adipocytes was measured at the indicated time points following treatment with 1000 ng/mL A. baumannii-LPS (ATCC 19606), 1000 ng/mL E. coli-LPS (O111:B4), or saline. All experiments were performed in triplicates, and the results are shown as the mean ± SD. (compared to cells without LPS treatment). ; (compared to adipocytes without LPS treatment).