miR-155 Promotes ox-LDL-Induced Autophagy in Human Umbilical Vein Endothelial Cells

Figure 5

miR-155 regulates LC3 and p62 expression. HUVECs were transfected with miR-155 mimic, miR-155 inhibitor, and mir-155NC and exposed to ox-LDL (100 μg/ml) for 6 h. Cells were harvested for qRT-PCR to detect the expression of mRNA and for Western blot to quantify the protein expression level. (a, c) qRT-PCR analysis of LC3 and p62. Results are the means ± SD of three separate experiments, . (b, d) Western blot analysis of LC3 and p62. Left panels present representative blots of LC3-II and p62; right bar graphs showed statistics of optical density measurements. Results are the means ± SD of three separate experiments, .

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