Regular Voluntary Exercise Potentiates Interleukin-1β and Interleukin-18 Secretion by Increasing Caspase-1 Expression in Murine Macrophages

Figure 5

Effects of regular voluntary exercise (VE) on the protein levels of prointerleukin- (IL-) 1β and pro-IL-18 in macrophages stimulated with or without lipopolysaccharide (LPS). Peritoneal-exudate macrophages isolated from the sedentary control (SC) and VE mice were cultured for 6 h in the presence or absence of 100 ng/mL LPS. (a) The protein levels of pro-IL-1β and pro-IL-18 were analyzed by western blotting. (b) The protein levels are presented as ratios relative to the levels of glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Mean ± standard error of the mean (SEM; ) values are provided. ; (by one-way analysis of variance [ANOVA] and the Bonferroni test).

(a)

(b)