Regular Voluntary Exercise Potentiates Interleukin-1β and Interleukin-18 Secretion by Increasing Caspase-1 Expression in Murine Macrophages

Figure 7

Effects of regular voluntary exercise (VE) on the expression levels of inflammasome components in macrophages. Immediately after peritoneal-exudate macrophages were isolated from the sedentary control (SC) and VE mice, whole cellular proteins and total cellular RNA were extracted. (a) The protein levels of Nod-like receptor family pyrin domain containing 3 (NLRP3) were analyzed by western blotting after immunoprecipitation (IP) (upper panel). The protein levels of apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) (b) and procaspase-1 (c) were analyzed by western blotting (upper panel). The protein levels are presented as ratios relative to the levels of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (lower panel). The mRNA levels of caspase-1 (d) and NLRP3 (e) were analyzed by real-time polymerase chain reaction (PCR). The mRNA levels are presented as ratios relative to the levels of 18S rRNA. Mean ± standard error of the mean (SEM; ) values are provided. (by Student’s -test).

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